Journal article

EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS-1 (HIV-1) ENVELOPE GENE-PRODUCTS TRANSCRIBED FROM A HETEROLOGOUS PROMOTER - KINETICS OF HIV-1 ENVELOPE PROCESSING IN TRANSFECTED CELLS

C BIRD, J BURKE, PA GLEESON, J MCCLUSKEY

JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 1990

Abstract

The expression of human immunodeficiency virus 1 (HIV-1) envelope glycoprotein products was studied in cells transfected with env gene constructs transcribed from an SV40 promoter. Gene constructs possessing the complete tat, rev (tat+ rev+) and env genes were transiently expressed in COS-1 cells as precursor SU-TM (gp160), SU.TM (gp120 x 41), and nucleolar rev protein. In addition, envelope glycoprotein was detected on the surface of those transfected COS-1 cells expressing abundant levels of env protein. Transfected constructs possessing a mutated tat translational initiation codon (tat-rev+) were expressed in COS-1 cells with at least a 10-fold increase in the level of envelope glycoprote..

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