Reduced neonatal regulatory T cell response to microbial stimuli associates with subsequent eczema in high-risk infants
Intan H Ismail, Robert J Boyle, Li-Jeen Mah, Paul V Licciardi, Mimi LK Tang
Pediatric Allergy and Immunology | WILEY-BLACKWELL | Published : 2014
BACKGROUND: Regulatory T cells (Treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. We evaluated cord blood Treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year. METHODS: Cord blood mononuclear cells from 72 neonates were cultured with toll-like receptors (TLR2) ligands: lipoteichoic acid (LTA) and heat-killed Lactobacillus rhamnosus GG (HKL); TLR4 ligand: lipopolysaccharide (LPS); ovalbumin (OVA); anti-CD3; or media for 48 h. Treg numbers and Treg cytokines were assessed in relat..View full abstract
The studies were funded by grants from the Jack Brockhoff Foundation, the Murdoch Children's Research Institute and the Ilhan Food Allergy Foundation. Intan Hakimah Ismail was supported by a scholarship from the Ministry of Higher Education, Malaysia and Universiti Putra Malaysia. Robert Boyle was supported by a University of Melbourne Baillieu Scholarship, a Murdoch Children's Research Institute Postgraduate Scholarship and a National Institute for Health Research Biomedical Research Centre. LGG and placebo capsules were manufactured and supplied by Dicofarm ltd (Roma, Italy). The Murdoch Childrens Research Institute receives infrastructure funding from the Victorian Government's Operational Infrastructure Support Programme.