Multimeric Recombinant M2e Protein-Based ELISA: A Significant Improvement in Differentiating Avian Influenza Infected Chickens from Vaccinated Ones
Farshid Hadifar, Jagoda Ignjatovic, Simson Tarigan, Risa Indriani, Esmaeil Ebrahimie, Noor Haliza Hasan, Andrea McWhorter, Sophie Putland, Abdulghaffar Ownagh, Farhid Hemmatzadeh
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2014
Killed avian influenza virus (AIV) vaccines have been used to control H5N1 infections in countries where the virus is endemic. Distinguishing vaccinated from naturally infected birds (DIVA) in such situations however, has become a major challenge. Recently, we introduced the recombinant ectodomain of the M2 protein (M2e) of H5N1 subtype as a novel tool for an ELISA based DIVA test. Despite being antigenic in natural infection the monomer form of the M2e used in ELISA had limited antigenicity and consequently poor diagnostic capability. To address this shortcoming, we evaluated the use of four tandem copies of M2e (tM2e) for increased efficiency of M2e antibody detection. The tM2e gene of H5N..View full abstract
Awarded by Australian Centre for International Agricultural Research (ACIAR)
This work was supported by Australian Centre for International Agricultural Research (ACIAR) fund (project number AH/2010/039) and the University of Adelaide post graduate support fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.