Monitoring of Strain-Dependent Responsiveness to TLR Activation in the Mouse Anterior Segment Using SD-OCT
Laura Elizabeth Downie, Matthew James Stainer, Holly Rose Chinnery
Investigative Ophthalmology & Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2014
PURPOSE: To determine whether spectral-domain optical coherence tomography (SD-OCT) can be used to longitudinally monitor inflammation in the mouse anterior segment and to identify any strain-dependent differences in responsiveness to distinct toll-like receptor (TLR) ligands. METHODS: Corneal inflammation was induced in BALB/c and C57BL/6 mice following central corneal abrasions and topical application of saline, TLR-4 ligand, lipopolysaccharide (LPS), or TLR-9 ligand, CpG-oligodeoxynucleotide (CpG-ODN; CpG). Anterior-segment images were captured using SD-OCT at baseline, 24 hours, and 1 week post treatment. Corneal thickness, stromal haze, and the number of keratic precipitates (KP) and an..View full abstract
Awarded by National Health and Medical Research Council of Australia
Supported by a National Health and Medical Research Council of Australia Project Grant (APP1042612).