Journal article

Enhanced amylolytic activity in germinating barley through synthesis of a bacterial Alpha-amylase

D Tull, BA Phillipson, B Kramhøft, S Knudsen, O Olsen, B Svensson

Journal of Cereal Science | Published : 2003

Abstract

Aleurone protoplasts were transfected to express synthetic genes encoding cytosolic and secreted forms of the alkalophilic Bacillus alpha-amylase, alkBA. The alpha-amylase activity in the cytosol of transfected protoplasts was increased 4 fold compared to the controls. Secreted alkBA was probably glycosylated, consistent with its higher apparent molecular mass, whereas the molecular mass of cytosolic alkBA was comparable to that of unglycosylated alkBA of bacterial origin. During micromashing, activity, was better retained for the secreted enzyme than for the cytosolic form. Subsequently, barley was transformed with the gene encoding the secreted form of alkBA. Out of eight selected transgen..

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University of Melbourne Researchers

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