Journal article
Assessment of sequence variability in a p23 gene region within and among three genotypes of the Theileria orientalis complex from south-eastern Australia
PK Perera, RB Gasser, A Jabbar
Ticks and Tick Borne Diseases | Published : 2015
Abstract
Oriental theileriosis is a tick-borne, protozoan disease of cattle caused by one or more genotypes of Theileria orientalis complex. In this study, we assessed sequence variability in a region of the 23. kDa piroplasm membrane protein (p23) gene within and among three T. orientalis genotypes (designated buffeli, chitose and ikeda) in south-eastern Australia. Genomic DNA (n=. 100) was extracted from blood of infected cattle from various locations endemic for oriental theileriosis and tested by polymerase chain reaction (PCR)-coupled mutation scanning (single-strand conformation polymorphism (SSCP)) and targeted sequencing analysis. Eight distinct sequences represented all DNA samples, and thre..
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Funding Acknowledgements
This project was partially supported by the Department of Agriculture Fisheries and Forestry (DAFF), Dairy Australia, a Collaborative Research Grant (the University of Melbourne) (A.J.) and the Australian Research Council (ARC) (R.B.G. et al.). Funding from the CASS Foundation and The Ian Potter Foundation is also gratefully acknowledged (A.J.). P.P. is a grateful recipient of the International Postgraduate Research Scholarship (IPRS) and Australian Postgraduate Award (APA) through The University of Melbourne.