Journal article
Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes
DG Gorasia, NL Dudek, PD Veith, R Shankar, H Safavi-Hemami, NA Williamson, EC Reynolds, MJ Hubbard, AW Purcell
Journal of Proteome Research | Published : 2015
DOI: 10.1021/pr500643h
Abstract
The complex interplay of many cell types and the temporal heterogeneity of pancreatic islet composition obscure the direct role of resident alpha and beta cells in the development of Type 1 diabetes. Therefore, in addition to studying islets isolated from non-obese diabetic mice, we analyzed homogeneous cell populations of murine alpha (αTC-1) and beta (NIT-1) cell lines to understand the role and differential survival of these two predominant islet cell populations. A total of 56 proteins in NIT-1 cells and 50 in αTC-1 cells were differentially expressed when exposed to proinflammatory cytokines. The major difference in the protein expression between cytokine-treated NIT-1 and αTC-1 cells w..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by a grant from the Juvenile Diabetes Research Foundation International (17-2012-134). A.W.P. acknowledges fellowship support from the Australian National Health and Medical Research Council. H.S.-H. is supported by a Marie Curie Fellowship from the European Union (CONBIOS 330486). M.J.H. acknowledges fellowship support from Melbourne Research Unit for Facial Disorders.