Differential PfEMP1 Expression Is Associated with Cerebral Malaria Pathology
Dumizulu L Tembo, Benjamin Nyoni, Rekah V Murikoli, Mavuto Mukaka, Danny A Milner, Matthew Berriman, Stephen J Rogerson, Terrie E Taylor, Malcolm E Molyneux, Wilson L Mandala, Alister G Craig, Jacqui Montgomery
PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2014
Plasmodium falciparum is unique among human malarias in its ability to sequester in post-capillary venules of host organs. The main variant antigens implicated are the P. falciparum erythrocyte membrane protein 1 (PfEMP1), which can be divided into three major groups (A-C). Our study was a unique examination of sequestered populations of parasites for genetic background and expression of PfEMP1 groups. We collected post-mortem tissue from twenty paediatric hosts with pathologically different forms of cerebral malaria (CM1 and CM2) and parasitaemic controls (PC) to directly examine sequestered populations of parasites in the brain, heart and gut. Use of two different techniques to investigate..View full abstract
Awarded by Wellcome Trust
Awarded by US National Institute of Health
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
JM was supported by a Wellcome Trust fellowship (www.wellcome.ac.uk; grant 080964) and DLT by a Malawi-Liverpool-Wellcome Trust studentship. This work was supported by The Wellcome Trust (071376 and 098051) and US National Institute of Health (www.nih.gov; grant 5R01AI034969). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.