A novel role for the Pol I transcription factor UBTF in maintaining genome stability through the regulation of highly transcribed Pol II genes
Elaine Sanij, Jeannine Diesch, Analia Lesmana, Gretchen Poortinga, Nadine Hein, Grace Lidgerwood, Donald P Cameron, Jason Ellul, Gregory J Goodall, Lee H Wong, Amardeep S Dhillon, Nourdine Hamdane, Lawrence I Rothblum, Richard B Pearson, Izhak Haviv, Tom Moss, Ross D Hannan
GENOME RESEARCH | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2015
Mechanisms to coordinate programs of highly transcribed genes required for cellular homeostasis and growth are unclear. Upstream binding transcription factor (UBTF, also called UBF) is thought to function exclusively in RNA polymerase I (Pol I)-specific transcription of the ribosomal genes. Here, we report that the two isoforms of UBTF (UBTF1/2) are also enriched at highly expressed Pol II-transcribed genes throughout the mouse genome. Further analysis of UBTF1/2 DNA binding in immortalized human epithelial cells and their isogenically matched transformed counterparts reveals an additional repertoire of UBTF1/2-bound genes involved in the regulation of cell cycle checkpoints and DNA damage r..View full abstract
Awarded by Canadian Institutes of Health Research (CIHR)
We thank Marcia Amalia for her technical contribution. This work was supported by grants from the National Health and Medical Research Council of Australia (NHMRC), the Leukaemia Foundation of Australia, Cancer Council Victoria, and the Captain Courageous Foundation. R.D.H. is supported by a NHMRC fellowship.T.M. is supported by funding from the Canadian Institutes of Health Research (CIHR) (#MOP12205, http://www. cihr-irsc.gc.ca/e/193.html).