Journal article
The GTPase regulatory proteins pix and git control tissue growth via the hippo pathway
LG Dent, CLC Poon, X Zhang, JL Degoutin, M Tipping, A Veraksa, KF Harvey
Current Biology | Published : 2015
Abstract
The Salvador-Warts-Hippo (Hippo) pathway is a conserved regulator of organ size and is deregulated in human cancers [1]. In epithelial tissues, the Hippo pathway is regulated by fundamental cell biological properties, such as polarity and adhesion, and coordinates these with tissue growth [2-4]. Despite its importance in disease, development, and regeneration, the complete set of proteins that regulate Hippo signaling remain undefined. To address this, we used proteomics to identify proteins that bind to the Hippo (Hpo) kinase. Prominent among these were PAK-interacting exchange factor (known as Pix or RtGEF) and G-protein-coupled receptor kinase-interacting protein (Git). Pix is a conserved..
View full abstractGrants
Awarded by Harvard Medical School
Funding Acknowledgements
We thank S. Bahri, B. Baum, A.P. Haghighi, Z-C Lai, E. Manser, N. Tapon, the Developmental Studies Hybridoma Bank, the Vienna Drosophila RNAi Center, the Australian Drosophila Research Support Facility (http://www.ozdros.com), and the Bloomington Drosophila Stock Centre for fly stocks, plasmids and antibodies. We thank Liu Yang for generating SAINT scores. K.F.H is a Sylvia and Charles Viertel Senior Medical Research Fellow. This research was supported by a Project Grant from the National Health and Medical Research Council of Australia, and by NIH grant GM097727 and NSF grant 0640700 to A.V. Mass spectrometry was performed at the Taplin Mass Spectrometry Facility, Harvard Medical School.