Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming
Pei Feng Choong, Hui Xin Teh, Hoon Koon Teoh, Han Kiat Ong, Kong Bung Choo, Shigeki Sugii, Soon Keng Cheong, Tunku Kamarul
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | IVYSPRING INT PUBL | Published : 2014
Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity ..View full abstract
Awarded by HIR-MoE
This research is supported by HIR-MoE Grant (Reference number - UM.C/625/1/HIR/MOHE/CHAN/03, account number - A000003-50001). The experiments were mainly carried out in the research laboratories at the Sungai Long Campus of the Universiti Tunku Abdul Rahman (UTAR).