Qualitative and quantitative analysis of fibrosis in the kidney
Tim D Hewitson, Edward R Smith, Chrishan S Samuel
Nephrology | WILEY | Published : 2014
Renal fibrosis results from an excess accumulation of connective tissue, primarily collagen, in response to tissue injury-associated aberrant wound healing, which is over-expressed in the renal vascular, glomerular and tubulointerstitial compartments. Despite being the final common pathway of end stage kidney disease, there is a lack of consensus on standardized approaches to measure fibrosis. In this article we therefore describe how a combination of immunohistochemical staining and biochemical measurement of hydroxyproline can be used to qualitatively and quantitatively examine the different forms of fibrosis. These techniques provide measures of both the composition of fibrosis, and a mea..View full abstract
Awarded by National Health & Medical Research Council (NHMRC) of Australia Senior Research Fellowship
C. S. Samuel is supported by a National Health & Medical Research Council (NHMRC) of Australia Senior Research Fellowship (APP1041766). All authors have contributed significantly, and are in agreement with the content of the manuscript. The authors have no financial arrangements with any company whose products figure in the manuscript, or with a company making a competing product. The manuscript has not been published elsewhere, and is not under consideration by any other publication.