Journal article

Geographic variation in the eukaryotic virome of human diarrhea

Lori R Holtz, Song Cao, Guoyan Zhao, Irma K Bauer, Donna M Denno, Eileen J Klein, Martin Antonio, O Cohn Stine, Thomas L Snelling, Carl D Kirkwood, David Wang

Virology | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2014

Abstract

Little is known about the population of eukaryotic viruses in the human gut ("virome") or the potential role it may play in disease. We used a metagenomic approach to define and compare the eukaryotic viromes in pediatric diarrhea cohorts from two locations (Melbourne and Northern Territory, Australia). We detected viruses known to cause diarrhea, non-pathogenic enteric viruses, viruses not associated with an enteric reservoir, viruses of plants, and novel viruses. Viromes from Northern Territory children contained more viral families per sample than viromes from Melbourne, which could be attributed largely to an increased number of sequences from the families Adenoviridae and Picornaviridae..

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Grants

Awarded by National Institutes of Health


Awarded by US Department of Agriculture


Awarded by Bill & Melinda Gates Foundation


Awarded by Children's Discovery Institute


Awarded by Morphology and Biobank Cores of the Washington University School of Medicine Digestive Diseases Research Core Center


Awarded by National Institutes of Health-National Center for Advancing Translational Sciences


Awarded by National Health and Medical Research Council, Australia


Awarded by Medical Research Council


Awarded by NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Funding Acknowledgements

This work was funded in part by National Institutes of Health [Grant U54 AI057160] to the Midwest Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research, US Department of Agriculture [Grant 0202238], Bill & Melinda Gates Foundation [OPP1016839], Children's Discovery Institute (MD-FR-2013-292), the Morphology and Biobank Cores of the Washington University School of Medicine Digestive Diseases Research Core Center [P30DK052574], and National Institutes of Health-National Center for Advancing Translational Sciences [UL1 TR000448 sub award KL2 TR000450 to L.R.H.]. D.W. holds an Investigator in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund. C.D.K. is supported by a Career Development Award fellowship from the National Health and Medical Research Council, Australia (607347).