Golgi-Located NTPDase1 of Leishmania major Is Required for Lipophosphoglycan Elongation and Normal Lesion Development whereas Secreted NTPDase2 Is Dispensable for Virulence
Fiona M Sansom, Julie E Ralton, M Fleur Sernee, Alice M Cohen, David J Hooker, Elizabeth L Hartland, Thomas Naderer, Malcolm J McConville
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2014
Parasitic protozoa, such as Leishmania species, are thought to express a number of surface and secreted nucleoside triphosphate diphosphohydrolases (NTPDases) which hydrolyze a broad range of nucleoside tri- and diphosphates. However, the functional significance of NTPDases in parasite virulence is poorly defined. The Leishmania major genome was found to contain two putative NTPDases, termed LmNTPDase1 and 2, with predicted NTPDase catalytic domains and either an N-terminal signal sequence and/or transmembrane domain, respectively. Expression of both proteins as C-terminal GFP fusion proteins revealed that LmNTPDase1 was exclusively targeted to the Golgi apparatus, while LmNTPDase2 was predo..View full abstract
Related Projects (1)
Awarded by NHMRC project
This work was funded by the Australian National Health and Medical Research Council (NHMRC; https://www.nhmrc.gov.au). FMS was supported by an NHMRC Postdoctoral Training fellowship and MJM is an NHMRC Principal Research Fellow. This work was supported by NHMRC project grant APP1059545. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.