Journal article

Enhancing Human Cardiomyocyte Differentiation from Induced Pluripotent Stem Cells with Trichostatin A

Shiang Y Lim, Priyadharshini Sivakumaran, Duncan E Crombie, Gregory J Dusting, Alice Pebay, Rodney J Dilley, K Turksen (ed.), A Nagy (ed.)

INDUCED PLURIPOTENT STEM (IPS) CELLS | HUMANA PRESS INC | Published : 2016

Abstract

Human induced pluripotent stem (iPS) cells are a promising source of autologous cardiomyocytes to repair and regenerate myocardium for treatment of heart disease. In this study, we describe a method for enhanced cardiomyocyte production from human iPS cells by treating embryoid bodies with a histone deacetylase inhibitor, trichostatin A (TSA), together with activin A and bone morphogenetic protein (BMP)-4. The resulting cardiomyocytes expressed cardiac-specific transcription factors and contractile proteins at both gene and protein levels. Functionally, the contractile embryoid bodies (EBs) displayed calcium cycling and were responsive to the chronotropic agents isoprenaline (0.1 μM) and car..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

These studies were supported by grants from the National Heart Foundation and National Health and Medical Research Council of Australia (1024817; 1056589). GJD is a Principal Research Fellow of NHMRC and AP is a Career Development Fellow of NHMRC. Support is also provided by the JR and JO Wicking Trust, Friedreich's Ataxia Research Association (research grant and 2012 Keith Michael Andrus Cardiac Research Award), Tony and Gwyneth Lennon Foundation, and the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.