Journal article

A microRNA expression atlas of mouse dendritic cell development

Timothy M Johanson, Marek Cmero, James Wettenhall, Andrew M Lew, Yifan Zhan, Mark MW Chong



Dendritic cells (DCs) are sentinel cells of the immune system and are essential for inducing a proper immune response. The mechanisms driving the development of DCs are not fully understood. Although the roles of cytokines and transcription factors have been a major focus, there is now substantial interest in the role of microRNAs (miRNAs). miRNAs are small RNAs that regulate gene expression by targeting messenger RNAs for translational repression and ultimately degradation. By means of deep sequencing, we have assembled a comprehensive and quantitative resource of miRNA expression during DC development. We show that mature DCs and their hematopoietic progenitors can be distinguished based o..

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Awarded by National Health and Medical Research Council, Australia

Awarded by Juvenile Diabetes Research Foundation

Awarded by Diabetes Australia Research Trust

Funding Acknowledgements

We thank Fiona Bell, Yuxia Zhang and Gaetano Naselli for assistance with library construction, Amit Kumar for assistance with data handling, and Catherine Yates for expert animal husbandry. This work was funded by grants from the National Health and Medical Research Council, Australia (#637338, #1004541 and #1042211 to MMWC; #637324 to YZ; #1037321, #1043414 and #637303 to AML), Juvenile Diabetes Research Foundation (5-2011-100 to MMWC) and the Diabetes Australia Research Trust (Y13G-CHOM to MMWC). MMWC is an Australian Research Council QEII Fellow, and AML is a Principal Research Fellow of the National Health and Medical Research Council, Australia. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian National Health and Medical Research Council Research Institute Infrastructure Support Scheme.