Journal article

Buffalo milk fat globules and their biological membrane: in situ structural investigations

Hanh TH Nguyen, Lydia Ong, Eric Beaucher, Marie-Noelle Madec, Sandra E Kentish, Sally L Gras, Christelle Lopez

Food Research International | Elsevier | Published : 2015


Milk fat globules and their surrounding biological membrane (the MFGM) are not well understood despite the importance of these milk components in human nutrition and the role of fat globules in determining the properties of dairy products. The objectives of this study were to investigate these unique colloidal assemblies and the microstructure of the MFGM in buffalo milk, which is the second largest global source of dairy products. In-situ structural investigations were performed at room temperature using confocal microscopy with multiple fluorescent probes (Nile Red, Rh-DOPE, the lectin WGA-488). Microscopic observations showed cytoplasmic crescents around fat globules and the heterogeneous..

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Funding Acknowledgements

Joelle Leonil, the head of INRA STLO (Rennes, France), is acknowledged for hosting Hanh Nguyen (PhD student). The authors thank Joel Guillemin (Cooperative de Bufflonnes, Maurs, France) for providing buffalo milks. The authors also acknowledge The Australian Government including The ARC Dairy Innovation Hub, The Rural Industries Research and Development Cooperation (RIRDC), The University of Melbourne, The Bio21 Molecular Science and Biotechnology Institute, The Particulate Fluids Processing Centre (PFPC) and The Clive Pratt Family for financial support.