PTEN secretion in exosomes
Ulrich Putz, Sophia Mah, Choo-Peng Goh, Ley-Hian Low, Jason Howitt, Seong-Seng Tan
METHODS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2015
PTEN was discovered as a membrane-associated tumor suppressor protein nearly two decades ago, but the concept that it can be secreted and taken up by recipient cells is revolutionary. Since then, various laboratories have reported that PTEN is indeed secreted and available for uptake by other cells in at least two different guises. First, PTEN may be packaged and exported within extracellular vesicles (EV) called exosomes. Second, PTEN may also be secreted as a naked protein in a longer isoform called PTEN-long. While the conditions favouring the secretion of PTEN-long remain unknown, PTEN secretion in exosomes is enhanced by the Ndfip1/Nedd4 ubiquitination system. In this report, we describ..View full abstract
This work was supported by the National Health and Medical Research Council (NH&MRC) and the Victorian Government through the Operational Infrastructure Scheme.