Journal article

Revisiting the role of histo-blood group antigens in rotavirus host-cell invasion

Raphael Boehm, Fiona E Fleming, Andrea Maggioni, Vi T Dang, Gavan Holloway, Barbara S Coulson, Mark von Itzstein, Thomas Haselhorst

NATURE COMMUNICATIONS | NATURE PUBLISHING GROUP | Published : 2015

Abstract

Histo-blood group antigens (HBGAs) have been proposed as rotavirus receptors. H type-1 and Lewis(b) antigens have been reported to bind VP8* from major human rotavirus genotypes P[4], P[6] and P[8], while VP8* from a rarer P[14] rotavirus recognizes A-type HBGAs. However, the role and significance of HBGA receptors in rotavirus pathogenesis remains uncertain. Here we report that P[14] rotavirus HAL1166 and the related P[9] human rotavirus K8 bind to A-type HBGAs, although neither virus engages the HBGA-specific α1,2-linked fucose moiety. Notably, human rotaviruses DS-1 (P[4]) and RV-3 (P[6]) also use A-type HBGAs for infection, with fucose involvement. However, human P[8] rotavirus Wa does n..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council for the award of an Australian Future Fellowship


Awarded by Australian Research Council


Awarded by National Health and Medical Research Council of Australia for the award of a Senior Research Fellowship


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

We are grateful to Enzo Palombo for the provision of HAL1166 rotavirus, Harry Greenberg for 1A10 antibody and Renee Winzar for technical assistance in protein production. T.H. thanks the Australian Research Council for the award of an Australian Future Fellowship (FT120100419). M.v.I. and B.S.C. also acknowledge the financial support of the Australian Research Council (DP1094393). B.S.C. is grateful to the National Health and Medical Research Council of Australia for the award of a Senior Research Fellowship (ID628319). M.v.I, B.S.C. and T.H. thank the National Health and Medical Research Council of Australia for a Project Grant (ID597439).