High-throughput imaging of heterogeneous cell organelles with an X-ray laser
Max F Hantke, Dirk Hasse, Filipe RNC Maia, Tomas Ekeberg, Katja John, Martin Svenda, N Duane Loh, Andrew V Martin, Nicusor Timneanu, Daniel SD Larsson, Gijs van der Schot, Gunilla H Carlsson, Margareta Ingelman, Jakob Andreasson, Daniel Westphal, Mengning Liang, Francesco Stellato, Daniel P DePonte, Robert Hartmann, Nils Kimmel Show all
Nature Photonics | NATURE PUBLISHING GROUP | Published : 2014
We overcome two of the most daunting challenges in single-particle diffractive imaging: collecting many high-quality diffraction patterns on a small amount of sample and separating components from mixed samples. We demonstrate this on carboxysomes, which are polyhedral cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12...min with the Linac Coherent Light Source running at 120...Hz. We separate different structures directly from the diffraction data and show that the size distribution is preserved during sample delivery. We automate phase retrieval and avoid recon..View full abstract
This work was supported by the Swedish Research Council, the Knut and Alice Wallenberg Foundation, the European Research Council, the Rontgen-Angstrom Cluster and Stiftelsen Olle Engkvist Byggmastare. Portions of this research were carried out at the Linac Coherent Light Source, a national user facility operated by Stanford University on behalf of the US Department of Energy, Office of Basic Energy Sciences. The authors thank the scientific and technical staff of the LCLS for support. The authors thank the CAMP collaboration for giving access to their experimental set-up and for supporting the experiment at the LCLS. The authors also acknowledge the Max Planck Society for funding the development and operation of the CAMP instrument.