Quantitative methodology is critical for assessing DNA methylation and impacts on correlation with patient outcome
Annette M Lim, Ida LM Candiloro, Nicholas Wong, Marnie Collins, Hongdo Do, Elena A Takano, Christopher Angel, Richard J Young, June Corry, David Wiesenfeld, Stephen Kleid, Elizabeth Sigston, Bernard Lyons, Danny Rischin, Benjamin Solomon, Alexander Dobrovic
Clinical Epigenetics | BMC | Published : 2014
BACKGROUND: DNA hypermethylation is reported as a frequent event and prognostic marker in head and neck squamous cell carcinomas (HNSCC). Methylation has been commonly assessed with non-quantitative methodologies, such as methylation-specific PCR (MSP). We investigated previously reported hypermethylated genes with quantitative methodology in oral tongue squamous cell carcinomas (OTSCC). RESULTS: The methylation status of 12 genes in 115 OTSCC samples was assessed by one or more of three quantitative analyses: methylation sensitive high resolution melting (MS-HRM), sensitive-melting analysis after real time-methylation specific PCR (SMART-MSP), and bisulfite pyrosequencing. In contrast to mu..View full abstract
A.M. Lim was supported by a Cancer Council of Victoria Postgraduate Cancer Research Scholarship.A. Dobrovic received funding from the National Breast Cancer Foundation, Cancer Australia and the Cancer Council of Victoria.