Journal article
Quantitative methodology is critical for assessing DNA methylation and impacts on correlation with patient outcome
AM Lim, ILM Candiloro, N Wong, M Collins, H Do, EA Takano, C Angel, RJ Young, J Corry, D Wiesenfeld, S Kleid, E Sigston, B Lyons, D Rischin, B Solomon, A Dobrovic
Clinical Epigenetics | BMC | Published : 2014
Open access
Abstract
Background: DNA hypermethylation is reported as a frequent event and prognostic marker in head and neck squamous cell carcinomas (HNSCC). Methylation has been commonly assessed with non-quantitative methodologies, such as methylation-specific PCR (MSP). We investigated previously reported hypermethylated genes with quantitative methodology in oral tongue squamous cell carcinomas (OTSCC). Results: The methylation status of 12 genes in 115 OTSCC samples was assessed by one or more of three quantitative analyses: methylation sensitive high resolution melting (MS-HRM), sensitive-melting analysis after real time-methylation specific PCR (SMART-MSP), and bisulfite pyrosequencing. In contrast to mu..
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Funding Acknowledgements
[ "A.M. Lim was supported by a Cancer Council of Victoria Postgraduate Cancer Research Scholarship.", "A. Dobrovic received funding from the National Breast Cancer Foundation, Cancer Australia and the Cancer Council of Victoria." ]