Journal article
Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism
F Cuskin, EC Lowe, MJ Temple, Y Zhu, EA Cameron, NA Pudlo, NT Porter, K Urs, AJ Thompson, A Cartmell, A Rogowski, BS Hamilton, R Chen, TJ Tolbert, K Piens, D Bracke, W Vervecken, Z Hakki, G Speciale, JL Munoz-Munoz Show all
Nature | Published : 2015
DOI: 10.1038/nature13995
Abstract
Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall a-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast a-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of a-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presen..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by grants from the European Research Council (G.J.D., Glycopoise; H.J.G., No. 322820), The Wellcome Trust (H.J.G., WT097907AIA), BBSRC (M.J.T., G.J.D.; BB/G016127/1), US Department of Energy (DOE) Bioenergy Research Center (BESC) supported by the Office of Biological and Environmental Research in the DOE Office of Science (M.J.P.) and the National Institutes of Health (E.C.M. and T.J.T., GM090080). Gnotobiotic mouse experiments were supported by a subsidy from the University of Michigan Medical School Host Microbiome Initiative, Agriculture and Agri-Food Canada, AgriFlex (D.W.A., #2510), Canadian Institute of Health Research operating grant (A.B.B., MOP-68913), Australian Research Council; Mizutani Foundation (S.J.W.). We thank the staff of the Diamond Light Source for the provision of beamline facilities. We would also like to thank various members of ICaMB for providing the yeast strains used in this work. We were greatly saddened by the passing of C.Z. during the course of this work.