Journal article

Dynamics of smoking-induced genome-wide methylation changes with time since smoking cessation

Florence Guida, Torkjel M Sandanger, Raphaele Castagne, Gianluca Campanella, Silvia Polidoro, Domenico Palli, Vittorio Krogh, Rosario Tumino, Carlotta Sacerdote, Salvatore Panico, Gianluca Severi, Soterios A Kyrtopoulos, Panagiotis Georgiadis, Roel CH Vermeulen, Eiliv Lund, Paolo Vineis, Marc Chadeau-Hyam



Several studies have recently identified strong epigenetic signals related to tobacco smoking. However, an aspect that did not receive much attention is the evolution of epigenetic changes with time since smoking cessation. We conducted a series of epigenome-wide association studies to capture the dynamics of smoking-induced epigenetic changes after smoking cessation, using genome-wide methylation profiles obtained from blood samples in 745 women from 2 European populations. Two distinct classes of CpG sites were identified: sites whose methylation reverts to levels typical of never smokers within decades after smoking cessation, and sites remaining differentially methylated, even more than ..

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Awarded by National Cancer Institute

Awarded by 7th European Framework Programme

Awarded by Fondation de France

Awarded by Fondation pour la Recherche Medicale (FRM)

Awarded by ERC

Funding Acknowledgements

This study was supported by the Human Genetics Foundation (HuGeF) and has been carried out within the Transdisciplinary Research in Cancer of the Lung (TRICL) project, supported by the National Cancer Institute, Grant U19 CA148127 02 to Christopher I. Amos. M.C.-H., S.A.K., R.C.H.V. and P.V. acknowledge the 7th European Framework Programme (Exposomics-308610-to P.V.). F.G. was supported by the Fondation de France (Grant 2012-00031604) and R.C. by the Fondation pour la Recherche Medicale (FRM, Grant number SPE20120523823). Gene expression and DNA methylation analyses in NOWAC samples were funded by ERC advanced grant Transcriptomics in cancer Epidemiology-ERC-2008-AdG-232997 and Tromso research foundation.