Journal article
Multiplex PCR for the detection and quantification of zoonotic taxa of Giardia, Cryptosporidium and Toxoplasma in wastewater and mussels
M Marangi, A Giangaspero, V Lacasella, A Lonigro, RB Gasser
Molecular and Cellular Probes | Published : 2015
Abstract
Giardia duodenalis, Cryptosporidium parvum and Toxoplasma gondii are important parasitic protists linked to water- and food-borne diseases. The accurate detection of these pathogens is central to the diagnosis, tracking, monitoring and surveillance of these protists in humans, animals and the environment. In this study, we established a multiplex real-time PCR (qPCR), coupled to high resolution melting (HRM) analysis, for the specific detection and quantification of each G.duodenalis (assemblage A), C.parvum and T.gondii (Type I). Once optimised, this assay was applied to the testing of samples (n=232) of treated wastewater and mussels (Mytilus galloprovincialis). Of 119 water samples, 28.6%..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
This work was partly supported by a grant from PON Puglia ERDF 2007-2013, Axis I, Line 1.2. Accordo di Programma Quadro. Reti di Laboratori Pubblici di Ricerca. Progetto L.A.I.F.F. (codice n. 47), Italy, and by another grant from PON REC 01_01480 IN.TE.R.R.A. (Technology and process innovations for irrigation reuse of treated municipal and agro-industrial wastewaters to achieve sustainable water resources management), Bari, Italy. The authors thank Dr Tiziana Caradonna for technical assistance. RBG's research was supported by the Australian Research Council (ARC), the National Health and Medical Research Council (NHMRC) of Australia, Melbourne Water Corporation and Yourgene Bioscience; it was also supported by a Victorian Life Sciences Computation Initiative (VLSCI; grant number VR0007) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government.