Cardiopulmonary bypass changes the plasma proteome in children undergoing tetralogy of Fallot repair

M Hepponstall; V Ignjatovic; S Binos; C Attard; V Karlaftis; Y Dudekem; P Monagle; IE Konstantinov

Journal article

Cardiopulmonary bypass changes the plasma proteome in children undergoing tetralogy of Fallot repair

M Hepponstall, V Ignjatovic, S Binos, C Attard, V Karlaftis, Y Dudekem, P Monagle, IE Konstantinov

Perfusion United Kingdom | Published : 2015

DOI: 10.1177/0267659114566065

Abstract

Introduction: Cardiopulmonary bypass (CPB) can be associated with deleterious clinical effects. However, the impact of CPB on inflammatory, immunological and other homeostatic pathways remains poorly understood. We investigated the impact of CPB on the plasma proteome in children undergoing tetralogy of Fallot repair. Methods: Blood samples were taken from 20 children prior to and at the end of CPB and 6h, 12h and 24h after CPB. Plasma was analysed by liquid chromatography-mass spectrometry (LC-MS) in a label-free, untargeted approach. Data were analysed using Genedata software to identify peptides that were differentially expressed (p<0.01 above a false discovery rate). Proteins were identi..

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University of Melbourne Researchers

Vera Ignjatovic Author

Vicky Karlaftis Author

Paul Monagle Author

Igor Konstantinov Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This project was funded by the National Health and Medical Research Council (NHMRC) grant (# 628756) and NHMRC postgraduate scholarship (# 1017734). This study was supported by the Victorian Government's Operational Infrastructure Support Program.