Cardiopulmonary bypass changes the plasma proteome in children undergoing tetralogy of Fallot repair
M Hepponstall, V Ignjatovic, S Binos, C Attard, V Karlaftis, Y d'Udekem, P Monagle, IE Konstantinov
PERFUSION-UK | SAGE PUBLICATIONS LTD | Published : 2015
INTRODUCTION: Cardiopulmonary bypass (CPB) can be associated with deleterious clinical effects. However, the impact of CPB on inflammatory, immunological and other homeostatic pathways remains poorly understood. We investigated the impact of CPB on the plasma proteome in children undergoing tetralogy of Fallot repair. METHODS: Blood samples were taken from 20 children prior to and at the end of CPB and 6h, 12h and 24h after CPB. Plasma was analysed by liquid chromatography-mass spectrometry (LC-MS) in a label-free, untargeted approach. Data were analysed using Genedata software to identify peptides that were differentially expressed (p<0.01 above a false discovery rate). Proteins were identi..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC)
Awarded by NHMRC
This project was funded by the National Health and Medical Research Council (NHMRC) grant (# 628756) and NHMRC postgraduate scholarship (# 1017734). This study was supported by the Victorian Government's Operational Infrastructure Support Program.