Journal article

Limisphaera ngatamarikiensis gen. nov., sp nov., a thermophilic, pink-pigmented coccus isolated from subaqueous mud of a geothermal hotspring

Heike Anders, Jean F Power, Andrew D MacKenzie, Kirill Lagutin, Mikhail Vyssotski, Eric Hanssen, John W Moreau, Matthew B Stott

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2015

Abstract

A novel bacterial strain, NGM72.4(T), was isolated from a hot spring in the Ngatamariki geothermal field, New Zealand. Phylogenetic analysis based on 16S rRNA gene sequences grouped it into the phylum Verrucomicrobia and class level group 3 (also known as OPB35 soil group). NGM72.4(T) stained Gram-negative, and was catalase- and oxidase-positive. Cells were small cocci, 0.5-0.8 µm in diameter, which were motile by means of single flagella. Transmission electron micrograph (TEM) imaging showed an unusual pirellulosome-like intracytoplasmic membrane. The peptidoglycan content was very small with only trace levels of diaminopimelic acid detected. No peptidoglycan structure was visible in TEM im..

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Grants

Funding Acknowledgements

Support for this work was provided by Geothermal Resources of New Zealand (GRN) Programme at GNS Science. Ngati Tahu-Ngati Whaoa are acknowledged as the having mana whenau (customary rights) over this strain. We also thank the Ngati Tahu Ngati Whaoa Runanga Trust for their enthusiasm for our research, and assistance in access and sampling of the Ngatamariki geothermal features, and naming this micro-organism.