Journal article

Comparison of Test Specificities of Commercial Antigen-Based Assays and In-House PCR Methods for Detection of Rotavirus in Stool Specimens

S Ye, SB Lambert, K Grimwood, S Roczo-Farkas, GR Nimmo, TP Sloots, CD Kirkwood, DM Whiley

JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2015

Abstract

Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.

Grants

Funding Acknowledgements

Funding for this study was provided by the Study, Education & Research Trust Fund, Pathology Queensland. S.B.L. is supported by a National Health and Medical Research Council (NHMRC) early career fellowship and a Children's Hospital Foundation (CHF) People Support Fellowship. Thermo Fisher and Abacus ALS provided the ProSpecT and Savyon kits, respectively, at a reduced rate for use in this study.