Comparison of Test Specificities of Commercial Antigen-Based Assays and In-House PCR Methods for Detection of Rotavirus in Stool Specimens
S Ye, SB Lambert, K Grimwood, S Roczo-Farkas, GR Nimmo, TP Sloots, CD Kirkwood, DM Whiley
JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2015
Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection.
Funding for this study was provided by the Study, Education & Research Trust Fund, Pathology Queensland. S.B.L. is supported by a National Health and Medical Research Council (NHMRC) early career fellowship and a Children's Hospital Foundation (CHF) People Support Fellowship. Thermo Fisher and Abacus ALS provided the ProSpecT and Savyon kits, respectively, at a reduced rate for use in this study.