Journal article

Pro-inflammatory mediators increase levels of the noncoding RNA GAS5 in airway smooth muscle and epithelial cells

Christine R Keenan, Michael J Schuliga, Alastair G Stewart

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | Published : 2015

Abstract

The long noncoding RNA (lncRNA) GAS5 has been found to act as a decoy for the glucocorticoid receptor (GR), thus implicating GAS5 as a potential regulator of glucocorticoid sensitivity and resistance. Airway smooth muscle (ASM) cells and airway epithelial cells (AEC) play an important role in the pathogenesis and persistence of asthma and other chronic airways diseases. These airway structural cell types are also important cellular targets of the anti-inflammatory actions of glucocorticoids. In this study, we sought to examine the relevance of GAS5 to glucocorticoid sensitivity and resistance in ASM and AEC. We provide the first evidence that pro-inflammatory mediators up-regulate GAS5 level..

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Grants

Awarded by National Health and Medical Research Council (Australia)


Funding Acknowledgements

The authors thank the Departments of Respiratory Medicine, Surgery, and Anatomical Pathology at the Alfred Hospital and Professor Catriona MacClean for assistance in obtaining human biopsies. This work was supported by the National Health and Medical Research Council (Australia) project grant No. 628691. Conflict of interest: All of the authors declare that they have no conflict of interest associated with this study.