Journal article

Recording, labeling, and transfection of single neurons in deep brain structures

Bowen Dempsey, Anita J Turner, Sheng Le, Qi-Jian Sun, Lama Bou Farah, Andrew M Allen, Ann K Goodchild, Simon McMullan

PHYSIOLOGICAL REPORTS | WILEY | Published : 2015

Abstract

Genetic tools that permit functional or connectomic analysis of neuronal circuits are rapidly transforming neuroscience. The key to deployment of such tools is selective transfection of target neurons, but to date this has largely been achieved using transgenic animals or viral vectors that transduce subpopulations of cells chosen according to anatomical rather than functional criteria. Here, we combine single-cell transfection with conventional electrophysiological recording techniques, resulting in three novel protocols that can be used for reliable delivery of conventional dyes or genetic material in vitro and in vivo. We report that techniques based on single cell electroporation yield r..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Awarded by National Health & Medical Research Council of Australia


Funding Acknowledgements

This work was funded by Macquarie University, the Australian Research Council (DP120100920) and National Health & Medical Research Council of Australia (604002).