Netrins and Frazzled/DCC promote the migration and mesenchymal to epithelial transition of Drosophila midgut cells
Melissa Pert, Miao Gan, Robert Saint, Michael J Murray
BIOLOGY OPEN | COMPANY OF BIOLOGISTS LTD | Published : 2015
Mesenchymal-epithelial transitions (METs) are important in both development and the growth of secondary tumours. Although the molecular basis for epithelial polarity is well studied, less is known about the cues that induce MET. Here we show that Netrins, well known as chemotropic guidance factors, provide a basal polarising cue during the Drosophila midgut MET. Both netrinA and netrinB are expressed in the visceral mesoderm, the substrate upon which midgut cells migrate, while their receptor frazzled (fra) is expressed in midgut cells. Netrins are required to polarise Fra to the basal surface, and Netrins and Fra undergo mutually-dependent endocytosis, with Fra subsequently trafficking to l..View full abstract
Awarded by ARC
This work was supported by an ARC Discovery grant [DP120104443 to R. Saint]; and a University of Melbourne RGSS grant to M. J. Murray.