Differential expression of the LYL, SCL and E2A helix-loop-helix genes within the hemopoietic system

Abstract

The helix-loop-helix genes LYL, SCL and E2,4 are associated with chromosome translocations found in human lymphoid leukemias. To establish their hematopoietic expression patterns, we have isolated murine LYL and SCL cDNA clones and investigated the expression of all three genes by Northern blot analysis of 58 murine hemopoietic cell lines and tissues. The nucleotide sequences of LYL cDNA clones revealed alternative 5′ untranslated sequences and differential splicing within the 5′ portion of the coding region that may produce a LYL polypeptide lacking an N-terminal segment. The LYL gene was expressed in most myeloid, erythroid and B lymphocyte cell lines and displayed two alternative size cla..