Journal article

Galanin is an Autocrine Myelin and Oligodendrocyte Trophic Signal Induced by Leukemia Inhibitory Factor

Melissa M Gresle, Helmut Butzkueven, Victoria M Perreau, Anna Jonas, Junhua Xiao, Stefan Thiem, Fiona E Holmes, William Doherty, Pik-Ying Soo, Michele D Binder, Rainer Akkermann, Vilija G Jokubaitis, Holly S Cate, Mark P Marriott, Andrew L Gundlach, David Wynick, Trevor J Kilpatrick

GLIA | WILEY-BLACKWELL | Published : 2015

Abstract

In order to further investigate the molecular mechanisms that regulate oligodendrocyte (OC) survival, we utilized microarrays to characterize changes in OC gene expression after exposure to the cytokines neurotrophin3, insulin, or leukemia inhibitory factor (LIF) in vitro. We identified and validated the induction and secretion of the neuropeptide galanin in OCs, specifically in response to LIF. We next established that galanin is an OC survival factor and showed that autocrine or paracrine galanin secretion mediates LIF-induced OC survival in vitro. We also revealed that galanin is up-regulated in OCs in the cuprizone model of central demyelination, and that oligodendroglial galanin express..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) of Australia


Awarded by US National Multiple Sclerosis Society


Awarded by NHMRC


Awarded by NHMRC Centre for Research Excellence Grant


Awarded by National Institute on Aging


Awarded by Diabetes UK


Awarded by Medical Research Council


Awarded by NATIONAL INSTITUTE ON AGING


Awarded by MRC


Funding Acknowledgements

Grant sponsor: National Health and Medical Research Council (NHMRC) of Australia Career Development Fellowship (to H.B); Grant number: 628856; Grant sponsor: US National Multiple Sclerosis Society Project Grant; Grant number: RG 3850A3/1 (to HB, MPM, ALG, TJK); Grant sponsor: NHMRC Project Grant; Grant number: 454581 (to HB, ALG); Grant sponsor: NHMRC Centre for Research Excellence Grant (support for MMG); Grant number: 1001216; Grant sponsor: The Medical Research Council, The Wellcome Trust, Diabetes UK and National Institute on Aging (to DW); Grant number: AG10668; Grant sponsor: The Victorian Government Operational Infrastructure Support Programme.