A crucial role for the homeodomain transcription factor Hhex in lymphopoiesis
Jacob T Jackson, Chayanica Nasa, Wei Shi, Nicholas D Huntington, Clifford W Bogue, Warren S Alexander, Matthew P McCormack
Blood | AMER SOC HEMATOLOGY | Published : 2015
The hematopoietically expressed homeobox gene, Hhex, is a transcription factor that is important for development of definitive hematopoietic stem cells (HSCs) and B cells, and that causes T-cell leukemia when overexpressed. Here, we have used an Hhex inducible knockout mouse model to study the role of Hhex in adult hematopoiesis. We found that loss of Hhex was tolerated in HSCs and myeloid lineages, but resulted in a progressive loss of B lymphocytes in the circulation. This was accompanied by a complete loss of B-cell progenitors in the bone marrow and of transitional B-cell subsets in the spleen. In addition, transplantation and in vitro culture experiments demonstrated an almost complete ..View full abstract
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Awarded by Australian Government's National Health and Medical Research Council (NHMRC)
This work was supported by program (1016647, W.S.A.) and project grants (628386 and 1003391, M.P.M.), a fellowship (1058344, W.S.A.) and the Independent Research Institutes Infrastructure Support (IRIIS) Scheme from the Australian Government's National Health and Medical Research Council (NHMRC), grants-in-aid from the Cancer Council of Victoria and the Leukaemia Foundation of Australia (M.P.M.), a Future Fellowship from the Australian Research Council (M.P.M.), the Australia Cancer Research Fund (W.S.A.), and a Victorian State Government Operational Infrastructure Support (OIS) grant.