Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
M-A Sani, F Separovic
JOURNAL OF MAGNETIC RESONANCE | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2015
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid bilayers using oriented bilayers and magic angle spinning techniques. In particular, Boltzmann statistics REDOR and maximum entropy analysis of spinning side bands are used to analyse systems where multiple states of peptide or lipid molecules may co-exist. We propose that in future, rather than model membranes, structural studies in whole cells are feasibl..View full abstract
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Awarded by Australian Research Council Grant
FS is grateful to the Australian Research Council Grant DP140102127 for financial support.