EXPRESSION OF PARASITE ANTIGENS IN ESCHERICHIA-COLI

Abstract

Immunological detection of cloned parasite antigens in E. coli offers an efficient means of identifying parasite antigens which may be relevant to immunity. Moreover, this technique enables the sequence of an antigen to be determined and also enables purification of the recombinant protein from bacterial lysates. The variety of different vectors available means that specific vectors are available for most purposes. Useful vectors are available which have not yet been employed to study parasites. Lambda phage has been extensively used while other phage vector systems have not, even though they could offer advantages over existing systems. Cloning in the M13 vector for example, allows direct s..