Criteria for dendritic cell receptor selection for efficient antibody-targeted vaccination

A Reuter; SE Panozza; C Macri; C Dumont; J Li; H Liu; E Segura; J Vega-Ramos; N Gupta; I Caminschi; JA Villadangos; APR Johnston; JD Mintern

Journal article

Criteria for dendritic cell receptor selection for efficient antibody-targeted vaccination

A Reuter, SE Panozza, C Macri, C Dumont, J Li, H Liu, E Segura, J Vega-Ramos, N Gupta, I Caminschi, JA Villadangos, APR Johnston, JD Mintern

Journal of Immunology | Published : 2015

DOI: 10.4049/jimmunol.1402535

Abstract

Ab-targeted vaccination involves targeting a receptor of choice expressed by dendritic cells (DCs) with Ag-coupled Abs. Currently, there is little consensus as to which criteria determine receptor selection to ensure superior Ag presentation and immunity. In this study, we investigated parameters of DC receptor internalization and determined how they impact Ag presentation outcomes. First, using mixed bone marrow chimeras, we established that Ag-targeted, but not nontargeted, DCs are responsible for Ag presentation in settings of Ab-targeted vaccination in vivo. Next, we analyzed parameters of DEC205 (CD205), Clec9A, CD11c, CD11b, and CD40 endocytosis and obtained quantitative measurements o..

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University of Melbourne Researchers

Dr CHRISTOPHE MACRI Author

Dr Nishma Gupta Author

Prof Jose Villadangos Author

Prof Justine Mintern Author

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Grants

Awarded by National Health and Medical Research Council of Australia Project Grant

Awarded by Australian Research Council Future Fellowship

Awarded by Agence National de la Recherche Grants

Awarded by Centre d'Investigation Clinique Institut Gustave Roussy-Curie

Awarded by Australian Research Council

Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

This work was supported by National Health and Medical Research Council of Australia Project Grant 1062549 (to A.P.R.J. and J.D.M.), Australian Research Council Future Fellowship FT110100265 (to A.P.R.J.), a Monash Larkins Fellowship (to A.P.R.J.), Agence National de la Recherche Grants ANR-10-IDEX-0001-02PSL* (to E.S.), ANR-11-LABX-0043 (to E.S.), and Centre d'Investigation Clinique Institut Gustave Roussy-Curie 1428 (Center of Clinical Investigation) (to E.S.), and by INSERM (to E.S.).