Journal article

Soluble NSF attachment protein receptor molecular mimicry by a Legionella pneumophilaDot/Icm effector

Nathan P King, Patrice Newton, Ralf Schuelein, Darren L Brown, Marketa Petru, Vojtech Zarsky, Pavel Dolezal, Lin Luo, Andrea Bugarcic, Amanda C Stanley, Rachael Z Murray, Brett M Collins, Rohan D Teasdale, Elizabeth L Hartland, Jennifer L Stow



Upon infection, Legionella pneumophila uses the Dot/Icm type IV secretion system to translocate effector proteins from the Legionella-containing vacuole (LCV) into the host cell cytoplasm. The effectors target a wide array of host cellular processes that aid LCV biogenesis, including the manipulation of membrane trafficking. In this study, we used a hidden Markov model screen to identify two novel, non-eukaryotic soluble NSF attachment protein receptor (SNARE) homologs: the bacterial Legionella SNARE effector A (LseA) and viral SNARE homolog A proteins. We characterized LseA as a Dot/Icm effector of L. pneumophila, which has close homology to the Qc-SNARE subfamily. The lseA gene was present..

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Awarded by Czech Science Foundation

Awarded by Charles University Grant Agency

Funding Acknowledgements

We thank Tatiana Khromykh, Nicholas Condon and Oleksiy Kovtun for valuable technical assistance and Kirill Alexandrov for provision of reagents. The authors acknowledge the facilities and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. This study made use of the Life Science Automation (LISA) and ACRF Cancer Biology Imaging Facilities at the IMB. This work was funded by the Australian National Health and Medical Research Council (NHMRC) Program in Cellular Microbiology (JLS, ELH, RDT), a fellowship from NHMRC (to JLS) and grants from the Czech Science Foundation (P305-10-0651) and the Charles University Grant Agency (573112).