Journal article

Differential effects of omega-conotoxin GVIA on cholinergic and noncholinergic secretomotor neurones in the guinea-pig small intestine

MA Vremec, JC Bornstein, CE Wright, A Humphrey

BRITISH JOURNAL OF PHARMACOLOGY | STOCKTON PRESS | Published : 1997

Abstract

1. Ussing chambers were used to study the effects of the specific N-type Ca2+ channel antagonist, omega-conotoxin GVIA, on neurally evoked secretion across isolated submucosa/mucosa preparations from the small intestine of the guinea-pig. 2. Cholinergic and non-cholinergic neurones were stimulated with 10 microM dimethylphenylpiperazinium (DMPP). Non-cholinergic secretomotor neurones were preferentially stimulated with 100 nM 5-hydroxytryptamine (5-HT), while cholinergic secretomotor neurones were preferentially stimulated with 3 microM 5-HT in the presence of the 5-HT2 receptor antagonist ketanserin (30 nM). 3. omega-Conotoxin GVIA (1 nM-1 microM) depressed the secretion evoked by DMPP in a..

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