Journal article

Hydrogen peroxide modulates Ca2 -activation of single permeabilized fibres from fast- and slow-twitch skeletal muscles of rats

DR Plant, GS Lynch, DA Williams

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY | KLUWER ACADEMIC PUBL | Published : 2000

Abstract

We examined the effects of redox modulation on single membrane-permeabilized fibre segments from the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus muscles of adult rats to determine whether the contractile apparatus was the redox target responsible for the increased contractility of muscles exposed to low concentrations of H2O2. The effects of H2O2 on maximum Ca2+-activated force were dose-dependent with 30 min exposure to 5 mM H2O2 causing a progressive decrease by 22+/-3 and 13+/-2% in soleus and EDL permeabilized muscle fibres, respectively. Lower concentrations of exogenous H2O2 (100 microM and 1 mM) had no effect on maximum Ca2+-activated force. Subsequent exposure ..

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University of Melbourne Researchers