Quantitative measurement of resting skeletal muscle [Ca2 ] following acute and long-term downhill running exercise in mice

Abstract

Alteration of resting free intracellular [Ca2+] ([Ca2+](i)) homeostasis has been implicated in the aetiology of skeletal muscle fibre injury following damaging pliometric (lengthening or 'eccentric') contractions. Quantitative measurements of resting [Ca2+](i) in skeletal muscles following acute or long-term exercise involving such injurious contractions have not been performed. We tested the hypothesis that, following an acute bout of pliometric exercise, the maximum force production (P(o)) of isolated skeletal muscles would be significantly reduced and that this deficit in force would be accompanied by an elevation in resting skeletal muscle [Ca2+](i). Further, we tested whether long-term ..