Journal article

A CHROMOSOME-9 DELETION IN PLASMODIUM-FALCIPARUM RESULTS IN LOSS OF CYTOADHERENCE

DJ KEMP, J THOMPSON, DA BARNES, T TRIGLIA, F KARAMALIS, C PETERSEN, GV BROWN, KP DAY

MEMORIAS DO INSTITUTO OSWALDO CRUZ | MEM INST OSWALDO CRUZ | Published : 1992

Abstract

Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting that PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in det..

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