Detection of QTL for metabolic and agronomic traits in wheat with adjustments for variation at genetic loci that affect plant phenology
Camilla B Hill, Julian D Taylor, James Edwards, Diane Mather, Peter Langridge, Antony Bacic, Ute Roessner
PLANT SCIENCE | ELSEVIER IRELAND LTD | Published : 2015
Mapping of quantitative trait loci associated with levels of individual metabolites (mQTL) was combined with the mapping of agronomic traits to investigate the genetic basis of variation and co-variation in metabolites, agronomic traits, and plant phenology in a field-grown bread wheat population. Metabolome analysis was performed using liquid chromatography-mass spectrometry resulting in identification of mainly polar compounds, including secondary metabolites. A total of 558 metabolic features were obtained from the flag leaves of 179 doubled haploid lines, of which 197 features were putatively identified, mostly as alkaloids, flavonoids and phenylpropanoids. Coordinated genetic control wa..View full abstract
The authors wish to thank the School of Botany (University of Melbourne) node of Metabolomics Australia (www.metabolomics.net.au) for access to the LC-MS facility, and Rudi Appels and Gabriel Keeble-Gagnere for useful comments and suggestions on the manuscript. This work was supported by the Australian Research Council and the Grains Research and Development Corporation; the South Australian Government; the University of Adelaide; the University of Queensland; the University of Melbourne; and by a Melbourne International Fee Remission Scholarship, a Melbourne International Research Scholarship, and a University of Melbourne Special Postgraduate Studentship to C.B.H.