Applicability of Next Generation Sequencing Technology in Microsatellite Instability Testing
Chun Gan, Clare Love, Victoria Beshay, Finlay Macrae, Stephen Fox, Paul Waring, Graham Taylor
Genes | MDPI | Published : 2015
Microsatellite instability (MSI) is a useful marker for risk assessment, prediction of chemotherapy responsiveness and prognosis in patients with colorectal cancer. Here, we describe a next generation sequencing approach for MSI testing using the MiSeq platform. Different from other MSI capturing strategies that are based on targeted gene capture, we utilize "deep resequencing", where we focus the sequencing on only the microsatellite regions of interest. We sequenced a series of 44 colorectal tumours with normal controls for five MSI loci (BAT25, BAT26, BAT34c4, D18S55, D5S346) and a second series of six colorectal tumours (no control) with two mononucleotide loci (BAT25, BAT26). In the fir..View full abstract
Grant support: Victorian Cancer Agency, National Health and Medical Research Council. Graham Taylor is supported by the Herman Trust. Chun Gan is supported by the University of Melbourne Research Grant.