Direct Transcriptional Reprogramming of Adult Cells to Embryonic Nephron Progenitors
Caroline E Hendry, Jessica M Vanslambrouck, Jessica Ineson, Norseha Suhaimi, Minoru Takasato, Fiona Rae, Melissa H Little
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | AMER SOC NEPHROLOGY | Published : 2013
Direct reprogramming involves the enforced re-expression of key transcription factors to redefine a cellular state. The nephron progenitor population of the embryonic kidney gives rise to all cells within the nephron other than the collecting duct through a mesenchyme-to-epithelial transition, but this population is exhausted around the time of birth. Here, we sought to identify the conditions under which adult proximal tubule cells could be directly transcriptionally reprogrammed to nephron progenitors. Using a combinatorial screen for lineage-instructive transcription factors, we identified a pool of six genes (SIX1, SIX2, OSR1, EYA1, HOXA11, and SNAI2) that activated a network of genes co..View full abstract
Awarded by Australian Stem Cell Centre
Awarded by National Health and Medical Research Council, Australia
This work was supported by Australian Stem Cell Centre Grant P067 and National Health and Medical Research Council, Australia Grant ID631362. Microscopy was performed at the Institute for Molecular Bioscience/Australian Cancer Research Foundation Cancer Biology Imaging Facility, which was established with the generous support of the Australian Cancer Research Foundation. C. E. H. is a Rosamond Siemon postgraduate scholar. M. H. L. is an National Health and Medical Research Council Senior Principal Research Fellow.