Proximal tubule overexpression of a locally acting IGF isoform, Igf-1Ea, increases inflammation after ischemic injury
Fiona K Rae, Norseha Suhaimi, Joan Li, Tommaso Nastasi, Esfir Slonimsky, Nadia Rosenthal, Melissa H Little
GROWTH HORMONE & IGF RESEARCH | CHURCHILL LIVINGSTONE | Published : 2012
OBJECTIVE: IGF-1 is an important regulator of postnatal growth in mammals. In mice, a non-circulating, locally acting isoform of IGF-1, IGF-1Ea, has been documented as a central regulator of muscle regeneration and has been shown to improve repair in the heart and skin. In this study, we examine whether local production of IGF1-Ea protein improves tubular repair after renal ischemia reperfusion injury. DESIGN: Transgenic mice in which the proximal-tubule specific promoter Sglt2 was driving the expression of an Igf-1Ea transgene. These animals were treated with an ischemic-reperfusion injury and the response at 24h and 5days compared with wildtype littermates. RESULTS: Transgenic mice demonst..View full abstract
We thank Bree A. Rumballe for her technical input and the EMBL Monterotondo Mouse Core for generation of the transgenic mouse lines. MHL is a Principal Research Fellow and FR is an Industry Fellow supported by the National Health and Medical Council, Australia. TM was supported. by a sponsored research agreement from Novartis Pharma AG.