Identification of Anchor Genes during Kidney Development Defines Ontological Relationships, Molecular Subcompartments and Regulatory Pathways
Rathi D Thiagarajan, Kylie M Georgas, Bree A Rumballe, Emmanuelle Lesieur, Han Sheng Chiu, Darrin Taylor, Dave TP Tang, Sean M Grimmond, Melissa H Little
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2011
The development of the mammalian kidney is well conserved from mouse to man. Despite considerable temporal and spatial data on gene expression in mammalian kidney development, primarily in rodent species, there is a paucity of genes whose expression is absolutely specific to a given anatomical compartment and/or developmental stage, defined here as 'anchor' genes. We previously generated an atlas of gene expression in the developing mouse kidney using microarray analysis of anatomical compartments collected via laser capture microdissection. Here, this data is further analysed to identify anchor genes via stringent bioinformatic filtering followed by high resolution section in situ hybridisa..View full abstract
Awarded by National Institutes of Health, USA
This work was funded by the National Institutes of Health, USA (NIDDK DK070136). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.