Comparative gene expression analysis of genital tubercle development reveals a putative appendicular Wnt7 network for the epidermal differentiation
Han Sheng Chiu, John C Szucsik, Kylie M Georgas, Julia L Jones, Bree A Rumballe, Dave Tang, Sean M Grimmond, Alfor G Lewis, Bruce J Aronow, James L Lessard, Melissa H Little
DEVELOPMENTAL BIOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2010
Here we describe the first detailed catalog of gene expression in the developing lower urinary tract (LUT), including epithelial and mesenchymal portions of the developing bladder, urogenital sinus, urethra, and genital tubercle (GT) at E13 and E14. Top compartment-specific genes implicated by the microarray data were validated using whole-mount in situ hybridization (ISH) over the entire LUT. To demonstrate the potential of this resource to implicate developmentally critical features, we focused on gene expression patterns and pathways in the sexually indeterminate, androgen-independent GT. GT expression patterns reinforced the proposed similarities between development of GT, limb, and cran..View full abstract
Awarded by National Institutes of Health
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
We thank Emmanuelle Lesieur for assistance in material collection and Jane Brennan, Jane Armstrong, Chris Armit, Jamie Davies, Derek Houghton, Sue Lloyd-MacGilp, Mehran Sharghi, Xinguin Pi, Yogmatee Roochun, Ying Cheng, Simon Harding, Jamie Davies, and Duncan Davidson for GUDMAP editorial and database support. We also thank Carol Wicking for scientific opinion on limb and facial development. ML is a Principal Research Fellow of the National Health and Medical Research Council. This work was supported by the National Institutes of Health via awards DK070136 (MHL) and DK070219 (JLL).