Journal article

Isolation of clonogenic, long-term self renewing embryonic renal stem cells

M Lusis, J Li, J Ineson, ME Christensen, A Rice, MH Little



A tissue stem cell should exhibit long-term self-renewal, clonogenicity and a capacity to differentiate into the tissue of origin. Such a postnatal renal stem cell has not been formally identified. The metanephric mesenchyme (MM) of the developing kidney gives rise to both the renal interstitium and the nephrons and is regarded as the progenitor population of the developing kidney. However, isolated MM does not self renew and requires immortalization for survival in culture. Here we report the isolation and sustained culture of long-term repopulating, clonal progenitors from the embryonic kidney as free floating nephrospheres. Such cells displayed clonal self renewal for in excess of twenty ..

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Funding Acknowledgements

The authors thank Dr. Thierry Gilbert for assistance with thin sections, Professor Kerry Atkinson and Dr Gary Brooke for the provision of murine MSC, Dr. Andreas Kispert for permission to access the NIH-3T3 Wnt4 expressing cell line and Dr. Ryuichi Nishinakamura for the provision of Sall1-GFP mice. We also acknowledge the input and advice of Professor Brent Reynolds and Dr. Rodney Rietze. ML is Principal Research Fellow of the National Health and Medical Research Council Fellow. This work was supported by the Australian Stem Cell Centre.