Journal article

Loss of renal microvascular integrity in postnatal Crim1 hypomorphic transgenic mice

Lorine Wilkinson, Thierry Gilbert, Arnold Sipos, Ildiko Toma, David J Pennisi, Janos Peti-Peterdi, Melissa H Little

KIDNEY INTERNATIONAL | ELSEVIER SCIENCE INC | Published : 2009

Abstract

Crim1 is a cell-surface, transmembrane protein that binds to a variety of cystine knot-containing growth factors, including vascular endothelial growth factor A. In the developing renal glomerulus, Crim1 acts to tether vascular endothelial growth factor A to the podocyte cell surface, thus regulating its release to glomerular endothelial cells. The hypomorphic transgenic mouse (Crim1(KST264/KST264)) has glomerular cysts and severe glomerular vascular defects because of the lack of functional Crim1 in the glomerulus. Adult transgenic mice have a reduced glomerular filtration rate and glomerular capillary defects. We now show that, in these adult transgenic mice, renal vascular defects are not..

View full abstract

Grants

Funding Acknowledgements

We acknowledge the support of the staff of the School of Biomedical Sciences Animal Facility, of the Transgenic Animal Service of Queensland (TASQ), the Queensland Histology Service, Queensland Institute of Medical Research, the Analytical Electron Microscopy Service, and of the Queensland University of Technology. We thank Tadashi Inagami of the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, USA, for the renin antibody. This study was funded by the National Health and Medical Research Council of Australia and TASQ is supported by the Australian Research Council SRC Centre for Functional and Applied Genomics.