Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney
Kylie Georgas, Bree Rumballe, Lorine Wilkinson, Han Sheng Chiu, Emmanuelle Lesieur, Thierry Gilbert, Melissa H Little
HISTOCHEMISTRY AND CELL BIOLOGY | SPRINGER | Published : 2008
The kidney is the most complex organ within the urogenital system. The adult mouse kidney contains in excess of 8,000 mature nephrons, each of which can be subdivided into a renal corpuscle and 14 distinct tubular segments. The histological complexity of this organ can make the clarification of the site of gene expression by in situ hybridisation difficult. We have defined a panel of seven antibodies capable of identifying the six stages of early nephron development, the tubular nephron segments and the components of the renal corpuscle within the embryonic and adult mouse kidney. We have analysed in detail the protein expression of Wt1, Calb1 Aqp1, Aqp2 and Umod using these antibodies. We h..View full abstract
Awarded by National Institutes of Health, USA
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
We would like to thank Darrin Taylor and Sean Grimmond for technical advice and assistance in designing the primers for the mRNA riboprobes. Images and annotated protein expression patterns using these antibodies are available on the GUDMAP gene expression database (http://www.gudmap.org). We would like to thank the Edinburgh GUDMAP Editorial Office. This work was supported by the National Institute of Diabetes Digestion and Kidney Diseases, National Institutes of Health, USA (DK070136-02).